This paper examines the forensic significance of toolmark evidence in establishing definitive physical links between perpetrators, their instruments, and criminal acts. Toolmarks classified as striations or impressions are created when a tool’s working surface alters a softer material, such as during a forced entry, wire cutting, or firearm discharge. The central principle of toolmark analysis is individualization: microscopic manufacturing defects and subsequent wear patterns on a tool create a unique, reproducible “toolmark signature.” Forensic examiners apply comparison microscopy to precisely analyze and compare the unique features found on the evidence mark (from the crime scene) with test marks generated by a suspect’s recovered tool. A positive match provides powerful corroborative evidence, directly linking a specific tool and by extension, its owner to the scene of the crime or to a series of connected incidents. Despite inherent challenges, modern techniques, including 3D profilometry and digital analysis, continually enhance the objectivity and reliability of this discipline. Ultimately, toolmark evidence plays a pivotal role in criminal investigations, often providing the crucial, non-circumstantial proof required to secure convictions and resolve cases.